recombinant tnf alpha

Mouse TNF-alpha Recombinant Protein Product RMTNFAI was serially diluted 14 in triplicate starting from a concentration of 100 ngmL in assay media containing 1 µgmL Actinomycin D 2X Gentamicin and 10 Horse Serum and 100 µL was added to each well. 1400 Liberty Ridge Drive Suite 101 Wayne PA 19087.

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. Recombinant Murine TNF-α is a soluble 156 amino acid protein 173 kDa which corresponds to C-terminal extracellular domain of the full length transmembrane protein. High-quality TNF-alpha proteins from ACROBiosystems. A triphasic controlled in vitro study using isolated tissue preparations treated in one of four ways.

Stable trimers are most common in solution. China Building 9 Jing Dongbei Technology Park No18 Kechuang 10th St BDA Beijing 100176 PRChina Sino Biological US Inc. TNF-alpha is synthesized as a 233 amino acid aa transmembrane protein mTNF-alpha with a theoretical molecular weight MW of 26 kDa 12 that forms a homotrimer.

Ad Human TNF alpha proteins - Expressed in HEK293 cells - Cited in 16 publications. MTNF-alpha is cleaved by TNF-alpha converting enzyme TACE and released in its 157 aa 17 kDa soluble form sTNF-alpha 1-5. The N-terminal amino acid is Val.

Ad Human TNF alpha proteins - Expressed in HEK293 cells - Cited in 16 publications. TNF-α binding to surface receptors elicits a wide array of biologic activities including. Ad Recombinant Protein Native Protein Cell Lysate GMP Protein for Choice.

TNF-α plays an important role in the immune response to microbial invasions and in the necrosis of specific tumors. TNF-α exists in two. Find recombinant tnf-alpha and related products for scientific research at MilliporeSigma.

To study the effect of human recombinant tumour necrosis factor alpha TNF alpha on contracture development and changes in the ionic composition of the external bathing solution of electrically stimulated rat hemidiaphragm preparations in vitro. Coli -derived recombinant protein corresponding to the C-terminal domain of human tumour necrosis factor alpha TNFa a pro-inflammatory cytokine with cytotoxic effects on various tumour cells. Many transformed cell lines also secrete TNF-α.

Products Building Blocks Explorer Technical Documents Site Content Papers Genes. Cell viability was measured after 19 hours of culture using a fluorometric assay method. Tumor Necrosis Factor-a Human Recombinant produced in EColi is a single non-glycosylated polypeptide chain containing 158 amino acids 157 aa.

TNF-α also plays a role in the induction of septic shock auto immune diseases rheumatoid arthritis inflammation and diabetes. Ad High bioactivity verified by cell based assay FACS ELISA SPR etc. Gain unique insights robust results with their unrivalled consistency specificity.

These TNF-alpha proteins are produced with different tags such as hFc Tag. Recombinant Human tumor necrosis factor alpha is an E. Tumor Necrosis Factor-alpha TNF-alpha is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

View more Product Details. TNF-α is secreted by macrophages monocytes neutrophils T-cells principally CD4 and NK-cells. Recombinant human TNF-alpha protein T-100 is a highly pure recombinant and biologically active protein.

The plate was incubated for 23 hours at 37C and then. A The biological activity of Mouse Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Ships Today 10 Product Category.

Recombinant mouse TNF-alpha 410-MT induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. TNF-α forms multimeric complexes. Recombinant Human Tumor Necrosis Factor alpha with HSA Tumor necrosis factor-alpha TNF-α is a pleiotropic inflammatory cytokine produced by several types of cells predominantly activated macrophages.

It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer which forms homotrimers in circulation. With high purity high biological activity high stability and other superior features you can use this Human TNF-alpha protein for relevant bioassay and related research. Molecular Mass The 157 amino acid recombinant protein has a predicted molecular mass of 17352 Da.

Of the mature human TNF-alpha and an N-terminal methionine and having a molecular mass of 175kDa. A 26 kD membrane form of TNF-α has also been described. Human TNF-α amino acids Val77-Leu233 Accession NM_000594 was expressed in E.

The TNF-alpha is purified by standard chromatographic techniques. Product Details Target Species Human Product Form Purified recombinant cytokine - lyophilized. See Related Products For research purposes only not for human use Applications Our bioassay Alomone Labs Recombinant human TNF-alpha protein induces cytolysis in HeLa cells.

High-quality TNF-alpha proteins from ACROBiosystems. The ED 50 for this effect is 8-50 pgmL. Recombinant Human TNF-alpha Protein Val77-Leu233 HPLC-verified 10602-HNAE is expressed in E.

TNF as also known as TNF-alpha or cachectin is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor TNF superfamily. GF023 is a recombinant polypeptide containing the mature form of human TNF-a with a polyhistidine tag at its amino terminal. Ad High bioactivity verified by cell based assay FACS ELISA SPR etc.

Gain unique insights robust results with their unrivalled consistency specificity. The mature form of human TNF-alpha has 157 amino acid residues. The DTT-reduced protein and non-reduced protein migrate at approximately 16kDa by SDS-PAGE.

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